Description
MXPr Abstract
Methoxpropamine (MXPr) is an arylcyclohexylamine dissociative drug with structural similarities with 3-MeO-PCE, ketamine and deschloroketamine.e undertook the molecular identification and characterization of in urine, hair and powder samples. We used a combination of several analytical methods: liquid-state nuclear magnetic resonance infra-red spectroscopy MXPr and liquid chromatography high-resolution mass spectrometry . The second objective was to explore the metabolism of MXPr in silico and in vitro. . This is the first report of the identification of in France with analytical findings. This study highlights the challenge of identifying new psychoactive substances (NPS) when they are missing from compound libraries and if a standard is not available. The use of various complementary analytical methods combined with HRMS offers a promising approach for the molecular characterization of NPS.
Introduction MXPr;
Methoxpropamine (MXPr or 3-MeO-2′-oxo-PCPr) is an arylcyclohexylamine dissociative drug and a higher homologue of methoxetamine that possesses structural similarities with 3-MeO-PCE, ketamine and deschloroketamine . differs from due to the replacement of ethylamino with propylamino. MXPr contains a chiral center; so two possible enantiomers of the substance may exist. . Like its analogs ketamine andacts as a potent antagonist of N-methyl-D-aspartate (NMDA) receptors . blocked NMDA receptor in a dose-dependent manner. The IC50 of MXPr and MXE decreased in the following order: (1.647 μM)> MXE (0.841 μM). Deaths due to MXE abuse have been reported and it is banned in many countries . The recreational use of MXE may induce tachycardia, confusion, agitation, ataxia, nystagmus and a dissociative state. Like abuse of is harmful to humans by blocking the NMDA receptors, so it has become a serious concern.
We report the first identification of in France with analytical findings. The primary aim was to identify and characterize in urine, hair and powder samples by combining several analytical methods: liquid state nuclear magnetic resonance (NMR), infrared (IR) spectroscopy and liquid chromatography high-resolution mass spectrometry (LC-HRMS). The second objective was to explore the metabolism of in silico and in vitro.
Chemicals and reagents
According to Richeval et al. , β-OH-ethyltheophyllin and methyl-clonazepam (internal standards), β-glucuronidase (Helix pomatia), alamethicin (Trichoderma viride), uridine diphosphate glucuronic acid (UDPGA), glucose-6-phosphate dehydrogenase (G6PD), glucose-6-phosphate (G6P), and 5-sulfosalicylic acid were all purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France).
Identification of MXPr powder
One-dimensional 1H NMR and two-dimensional 1H-13C and 1–1H NMR spectroscopy enabled the unambiguous identification of NMR resonances corresponding to the 16 carbon atoms of as well as the 12
Conclusion
This study highlights the difficulty of identifying some NPS when they are missing from the libraries and the standard is not available. Here we show that the combination of several analytical methods with HRMS is powerful for the molecular characterization of NPS. The identification of metabolites will improve the efficiency of toxicological screening libraries and widen the window for detecting in biological matrices in cases of MXPr intake. In view of the results of the present
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